Ion Exchange Column Diagram

Split stream and weak acid cation systems also reduce the total dissolved solids.

Ion exchange column diagram.

Ion exchange chromatography can be applied to the determination of ionic solutes such as inorganic anions cations transition metals and low molecular weight organic acids. They are well suited for processing feedwater for medium pressure boilers and for process water for the beverage industry. Thus the workflow below is given as a generalized iex workflow and particular running conditions for cation exchange chromatography may be adjusted to best suit your protein of interest the buffer system and the anion. Schematic diagram of the principles of chromatography as discovered by tswett 1901.

All ion exchange chromatography relies on electrostatic interactions between the resin functional groups and proteins of interest. Ion exchange dealkalization systems produce hardness free low alkalinity water at a reasonable cost and with a high degree of reliability. The interstitial liquid fed with the resin contains caustic soda and the liquor flow corresponds to one bed volume. Ion exchange ix systems are used across a variety of industries for water softening purification and separation purposes.

The hardness ions ca and mg move into the resin beads and each of these divalent. For example metal contaminants are undesirable due to their color taste odor or toxicity. Ion exchange takes place in one column while the resin in the second column is being regenerated resulting in continuous operation. After loading an impure protein sample onto an ion exchange chromatography column the column is washed to remove undesired proteins and other impurities and then the protein s of interest is eluted using either a salt gradient or a change in ph.

Key steps in the ion exchange chromatography procedure are listed below. The table below shows the material flows in m 3 h around the ion exchange column. The ion exchange column has a solid resin feed of 10 m 3 h and an interstitial liquid factor of 0 85. An impure protein sample is loaded into the ion exchange chromatography column at a particular ph.

Rohm and haas ion exchange ion exchange introduction 4 fd sep 2008 to soften water you take a cation exchange resin on which the mobile ion inside the beads is sodium na and you pass the hard water through a column filled with the sodium form resin. Cation exchange chromatography workflow. Ion exchange usually describes a processes of purification of aqueous solutions using solid polymeric ion exchange resin more precisely the term encompasses a large variety of processes where ions are exchanged between two electrolytes. Ion exchange chromatography workflow.

A separation column and a detector to measure the species eluted from the column.